Modular polyketide synthases: Investigating intermodular communication using 6 deoxyerythronolide B synthase module 2.
نویسندگان
چکیده
A novel variant of 6-deoxyerythronolide B synthase (DEBS) module 2 was constructed to explore the balance between protein-protein-mediated intermodular channeling and intrinsic substrate specificity within DEBS. This construct, termed (N3)Mod2+TE, was co-incubated with a complementary, donor form of the same module, (N5)Mod2(C2), as well as with a mutant of (N5)Mod2(C2) with an inactive ketosynthase domain, in order to determine the extent of intermediate channeling versus substrate diffusion into the downstream module.
منابع مشابه
Reprogramming a module of the 6-deoxyerythronolide B synthase for iterative chain elongation.
Multimodular polyketide synthases (PKSs) have an assembly line architecture in which a set of protein domains, known as a module, participates in one round of polyketide chain elongation and associated chemical modifications, after which the growing chain is translocated to the next PKS module. The ability to rationally reprogram these assembly lines to enable efficient synthesis of new polyket...
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عنوان ژورنال:
- Bioorganic & medicinal chemistry letters
دوره 16 1 شماره
صفحات -
تاریخ انتشار 2006